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We ablated porcine corneas with a free electron laser tuned to either 2.77 or 6.45μm, two matched wavelengths that predominantly target water and protein, respectively. The ejected nonvolatile debris and the crater left behind were examined by circular dichroism, Raman spectroscopy, and scanning electron microscopy to characterize the postablation conformation of collagen proteins. We found near-complete...
The fragment Aβ(25–35) of the Alzheimer's amyloid β -peptide, like its full-length peptide Aβ(1–42), has shown neurotoxic activities in cultured cells. The conformational preference of this important peptide is examined here in solution, gel, and film states (obtained with organic and aqueous solvents) by vibrational circular dichroism spectroscopy for the first time. For comparative studies, vibrational...
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