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Vector pMPM‐A4Ω and vectors pQE‐30 and pET‐45b(+) containing the 6x His‐tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non‐structural proteins in Escherichia coli. Coat protein (CP) and RNA‐dependent RNA polymerase (RdRp)–fragments RdRp43‐616 and RdRp304‐537 were chosen for expression. A high level of CP and RdRp304‐537 was obtained only in an expression system...
Polyclonal antibodies to recombinant Potato virus X (PVX) coat protein (PVX‐CP) were developed and their effectiveness determined in different ELISA protocols. The PVX‐CP gene was amplified by reverse transcription‐polymerase chain reaction, cloned and expressed in Escherichia coli. For immunization, the CP fractions from bacterial lysate were purified either by simple fractionation or by excision...
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