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Free‐living Naegleria fowleri causes primary amoebic meningoencephalitis (PAM) in humans and animals. To examine the effect of immunization with Nfa1 protein on experimental murine PAM because of N. fowleri, BALB/c mice were intra‐peritoneally or intra‐nasally immunized with a recombinant Nfa1 protein. We analysed Nfa1‐specific antibody and cytokine induction, and the mean survival time of infected...
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