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Tight junctions (TJ) are the anatomical component of blood‐testis (BTB) and blood‐epididymis (BEB) barriers and contain many proteins, including claudins. The presence of claudins in domestic cat testis and epididymis has not been previously described. This study aimed to determine whether claudin‐1 and claudin‐5 participate in the structure of BTB and BEB and whether their amounts differ between...