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During 3′ end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem loop by an endonuclease dependent on the U7 small nuclear ribonucleoprotein (snRNP). The upstream cleavage product corresponds to the mature histone mRNA, while the downstream product is degraded by a 5′-3′ exonuclease, also dependent on the U7 snRNP. To identify the two nuclease activities, we carried out...
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