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STIM1 and Orai1 have recently been identified to be crucial in the regulation of store-operated Ca 2+ entry. However, it remains to be established how STIM1 couples store depletion to the functioning of Orai1 in the plasma membrane. Using quantitative measurement, we find little STIM1 on the surface membrane which is not increased by store depletion. We further demonstrate that Orai1 assembles...
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