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We present a novel easy‐to‐operate and efficient method to improve the separation efficiency in short‐capillary electrophoresis by introducing steady backflow to counterbalance electro‐osmotic flow without the use of any external pressure. The backflow was easily generated by tapering the capillary end, which was achieved by heating a straight capillary and stretching it with a constant force. We...
Electrophoresis 2015, 36, 1549–1554. DOI: 10.1002/elps.201500013
Pressure‐driven backflow is an attractive method to improve CZE separation resolution via counterbalancing the EOF. In this study, we describe a new method to generate steady backflow in a short capillary via a tapered capillary tip, which can be easily fabricated by heating a straight capillary while stretching it with a constant force...
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