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Salmonella enterica, Staphylococcus aureus, and Shigella dysenteriae are the major water-borne and food-borne pathogens, and usually co-exist in very low numbers. Thus, a selective enrichment process is required for their simultaneous detection. Here, we report on the formulation of a selective enrichment broth, namely SSS broth, for the concurrent growth of Salmonella enterica serovar Enteritidis,...
A method combining multiplex real-time polymerase chain reaction (PCR) with high-resolution melting (HRM) analysis for rapid and specific simultaneous detection of Salmonella, Listeria monocytogenes, and Staphylococcus aureus was developed. The method included a melting-curve analysis of products and was evaluated by specificity, sensitivity and reproducibility analyses. Sensitivity and reproducibility...
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