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To develop a gold nanoparticle (AuNP) assay for direct detection of unamplified HURP RNA in urine.HURP RNA was extracted from urine samples (50 bladder carcinoma patients, 25 benign bladder lesions, and 25 controls) and further purified using magnetic nanoparticles (MNPs), functionalized with HURP RNA-specific oligonucleotides, and then detected by RT-PCR or gold nanoparticles.The developed HURP RNA...
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