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The role of uncoupling protein-2 (UCP-2) in β-cells is presently unclear. We have tested the notion that UCP-2 participates in β-cell defense against oxidants. Expression of the UCP-2 gene in clonal β-cells (INS-1) was decreased by 45% after 48 h of culture with vitamin E and selenite. When INS-1 cells were exposed to 200 μM H 2 O 2 for 5 min, the cell viability (MTT assay) decreased...
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