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This paper reports the biochemical and pharmacological characterization of a new myotoxic PLA 2 (EC 3.1.1.4) called PhTX-I, purified from Porthidium hyoprora venom by one step analytical chromatography reverse phase HPLC. The homogeneity of the PhTX-I fraction and its molecular mass were initially evaluated by SDS–PAGE and confirmed by MALDI-TOF spectrometry, indicating a molecular mass of...
We report the purification and biochemical/pharmacological characterization of two myotoxic PLA 2 (BbTX-II K49 PLA 2 homologue and BbTX-III PLA 2 ) from Bothrops brazili venom. Both were purified by a single chromatographic step on reverse phase HPLC, showing M r ∼14kDa for both myotoxins, showing high content of hydrophobic and basic amino acids as well as 14 half-cysteine...
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