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Much less attention has been paid to Zn2+ in artificial cerebrospinal fluid (ACSF), i.e., extracellular medium, used for in vitro slice experiments than divalent cations such as Ca2+. Approximately 2 mM Ca2+ is added to conventional ACSF from essentiality of Ca2+ signaling in neurons and glial cells. However, no Zn2+ is added to it, even though the importance of Zn2+ signaling in them is recognizing...
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