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Although an in vitro 3D environment cannot completely mimic the in vivo tumor site, embedding tumor cells in a 3D extracellular matrix (ECM) allows for the study of cancer cell behaviors and the screening of anti-metastatic reagents with a more in vivo-like context. Here we explored the behaviors of MDA-MB-231 breast cancer cells embedded in 3D collagen I. Diverse tumor environmental conditions (including...
Transmembrane 4L six family member 5 (TM4SF5) can regulate cell–cell adhesion and cellular morphology via cytoplasmic p27 Kip1 -mediated changes in RhoA activity. However, how TM4SF5 causes cytosolic p27 Kip1 stabilization remains unknown. In this study we found that TM4SF5-mediated Ser10 phosphorylation of p27 Kip1 required for cytosolic localization was not always correlated...
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