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The regulation of protein kinase C (PKC) isoforms by ceramide is still controversial. In this work, the yeast Saccharomyces cerevisiae was used as a model to elucidate the effect of ceramide on the activity of mammalian PKC isoforms. For that, isc1Δ cells, with a deletion in the pathway for ceramide production by hydrolysis of complex sphingolipids, individually expressing mammalian PKCα, δ and ζ...
Yeast revealed to be a versatile organism for studying endocytosis. Here, inhibition of endocytosis by H2O2 and its correlation with apoptotic cell death were ascertained in Saccharomyces cerevisiae. We found that H2O2 causes alterations in vacuolar morphology and a concentration‐dependent inhibition of endocytosis. We also found that H2O2‐induced endocytosis inhibition is a reversible process that...
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