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Fluorinated aromatic amino acids (FAAs) are promising tools when studying protein structure and dynamics by NMR spectroscopy. The incorporation FAAs in mammalian expression systems has been introduced only recently. Here, we investigate the effects of FAAs incorporation in proteins expressed in human cells, focusing on the probability of incorporation and its consequences on the 19F NMR spectra. By...
[4Fe‐4S]2+ cluster assembly in human cytosol requires both a [2Fe‐2S] cluster chaperone being able to donate two [2Fe‐2S]2+ clusters and an electron donor providing two electrons to reductively couple the two [2Fe‐2S]2+ clusters into a [4Fe‐4S]2+ cluster. The mechanism through which the cytosolic [4Fe‐4S]2+ cluster assembly works is still not defined. Here, we show that a hetero‐tetrameric complex...
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