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A new ratiometric fluorescence probe for hydrogen sulfide is developed. The design was based on a H2S-mediated diselenide cleavage and intramolecular cyclization to release the fluorophore. The probe showed high selectivity and sensitivity for H2S over other reactive sulfur species and was successfully applied for the detection of H2S in bovine plasma and bioimaging in live cells.
A novel fluorescent calcium indicator with a 490/582nm ratiometric emission has been designed and synthesized. The indicator exhibits a highly selective ratiometric emission response to Ca 2+ over other metal cations and a large Stokes shift of 202nm. Moreover, its practical cell imaging capability for intracellular Ca 2+ in the resting- and dynamic-state has been demonstrated in human...
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