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High‐throughput sequencing of pooled DNA was applied to polymorphism discovery in candidate genes involved in starch synthesis. This approach employed semi‐ to long‐range PCR (LR‐PCR) followed by next‐generation sequencing technology. A total of 17 rice starch synthesis genes encoding seven classes of enzymes, including ADP‐glucose pyrophosphorylase (AGPase), granule starch synthase (GBSS), soluble...
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