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Polyethylenimine (PEI) as a spacer-arm polymer was modified on amine-functioned Fe3O4 nanoparticles (Fe3O4–NH2 NPs). PEI modified Fe3O4 nanoparticles (Fe3O4–NH2–PEI NPs) were chelated with Cu2+ to immobilize laccase through metal affinity adsorption. Meanwhile, PEI un-modified Fe3O4–NH2 NPs acted as a control group. The adsorption capacity of Fe3O4–NH2–PEI–Cu2+ NPs was larger than Fe3O4–NH2–Cu2+ NPs...
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