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A new approach has been developed and used to minimize the timeand more carefully monitor and control the seed-train expansionprocess of recombinant mammalian cell lines. The process uses 50or 100 ml cryo-bags that contain frozen cells at high cell densities of 20 × 106 ml-1 (100 ml bags) or 40 × 106 cells ml-1 (50 ml bags). The frozen bag cell suspension is thawed and transferred directly into a...
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