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Macrophages are essential components of the innate immune system and crucial for pathogen elimination in early stages of infection. We previously observed that bone marrow-derived macrophages (BMMs) from C57BL/6 mice exhibited increased killing activity against Burkholderia pseudomallei compared to BMMs from BALB/c mice. This effect was particularly pronounced when cells were treated with IFN-γ. To...
The cold stress response of Pseudomonas putida KT2440 was investigated by genomewide deep cDNA sequencing and gel‐free MS‐based protein profiling. Transcriptome and proteome profiles were assessed at 30 °C and 2 h after a downshift from 30 to 10 °C. Pseudomonas putida adapted to lower ambient temperature by the activation of ribosome‐associated functional modules that facilitate translational efficiency...
Peptide mass fingerprinting by MALDI-MS and sequencing by tandem mass spectrometry have evolved into the major methods for identification of proteins following separation by two-dimensional gel electrophoresis, SDS–PAGE or liquid chromatography. One main technological goal of proteome analyses beside high sensitivity and automation was the comprehensive analysis of proteins. Therefore, the protein...
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