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The resorption pit assay is classically used to evaluate osteoclast activity on bone or dentine slices that can be eroded by these cells. Two different types of cells were generated from peripheral blood mononuclear cells cultured in the presence of M‐CSF + sRANKL or with M‐CSF + LPS. At the end of the culture period (21 days), cells were discarded and the dentine slices stained with toluidine blue...
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