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The exclusion of charged fluorescent dyes by intact cells has become a well‐established assay for determining viability of cells. In search for a noninvasive fluorescent probe capable of long‐term monitoring of cell death in real‐time, we evaluated a new anthracycline derivative DRAQ7. The novel probe does not penetrate the plasma membrane of living cells but when the membrane integrity is compromised,...
We outline a simple approach involving instrument setup and calibration for the analysis of Hoechst dye 33342‐loading in human cell lines for exploring heterogeneity in dye efflux efficiency and the status of side population (SP) A549 lung cancer cells. Dual excitation 488 nm/multiline UV (351–364 nm) flow cytometry was used to confirm ABCG2‐specific inhibition of dye efflux using Fumitremorgin C...
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