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Due to high infertility ratio nowadays, it is essential to explore efficient ways of enhancing mammalian reproductivity, in particular female reproductivity. Using female Oct4‐GFP embryonic stem cells, we mimic the in vivo development procedure to induce ES cells into epiblast cell‐like cells (EpiLCs) and then primordial germ cell‐like cells (PGCLCs). GFP positive PGCLCs that showed typical PGC markers...
DIXDC1 is a Dishevelled-Axin (DIX) domain-containing protein involved in neural development and Wnt signaling pathway. Besides the DIX domain, DIXDC1 also contains a coiled-coil domain (MTH domain), which is a common feature of centrosomal proteins. We have demonstrated that exogenously expressed GFP-tag fused DIXDC1 co-localize with γ-tubulin both at interphase and mitotic phase in HEK293 cells....
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