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AbstractThe gene lccK encoding a laccase of the white-rot basidiomycete Pleurotus ostreatus wild-type strain collected in Japan has been cloned, sequenced, and characterized. The isolated gene consists of 2929bp with the coding region interrupted by 19 introns and flanked by an upstream region in which putative CAAT and TATA elements were identified. Two putative N-glycosylation sites and four putative...
AbstractThree aryl alcohol oxidases (AAOs; EC 1.1.3.7) I, II, and III from the culture filtrate of a strain of white-rot fungus Pleurotus ostreatus were purified by multistep chromatography. Each of the purified AAOs I, II, and III had the same molecular masses of 70kDa and 72kDa on gel filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, respectively. Their optimum...
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