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Pseudomonas marginalis produced two pectate lyase isozymes in a medium containing sodium polypectate. Of the two, one had alkaline pI, 9.5, and the other had neutral pI, 7.0. Extracellular pectate lyase secretion started during mid-log phase and reached a maximum during the stationary phase of growth. Pectate lyase production was improved by the addition of 1 mM CaCl 2 which also enhanced...
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