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A 4D TROSY-based triple resonance experiment, 4D-HNCOi−1CAi, is presented which correlates intra-residue 1HN, 15N, 13 Cα chemical shifts with the carbonyl (13C′) shift of the preceding residue. The experiment is best used in concert with recently described 4D TROSY-HNCOCA and -HNCACO experiments [Yang, D. and Kay, L.E. (1999) J. Am. Chem. Soc., 121, 2571–2575]. In cases where degeneracy of (1HN,15...
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