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A diosgenin-rich plant, Costus speciosus (Koenig) Smith was found to contain β-glucosidase which converts a furostanol glycoside, protogracillin, to a spirostanol glycoside, gracillin. It was demonstrated that the enzyme was present in rhizomes of plants grown in a greenhouse and whole parts of plantlets cultured in vitro. The soluble enzyme from the cultivated rhizomes showed pH optima of 5.0 and...
In plants, spirostanol glycosides (steroid saponins) are known to be formed from furostanol glycosides during postharvest treatment and storage. Furostanol glycoside 26-O-β-glucosidase (F26G) involved in this conversion was purified to apparent homogeneity for the first time from Costus speciosus rhizomes which accumulate these glycosides. The enzyme was highly specific for cleavage of the C-26-bound...
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