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A sialidase capable of cleaving a glycoconjugate to yield 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (KDN) is isolated from hepatopancreas of mollusks. Preferably, the sialidase is isolated by processing crude oyster hepatopancreas to form a crude extract of the sialidase enzyme. The crude enzyme is further concentrated and refined utilizing sequential column chromatography fractogel EMD DEAE-650...
A sialidase capable of cleaving a glycoconjugate to yield 3-deoxy-D-glycero-D-galacto-2-nonulosonic acid (KDN) is isolated from hepatopancreas of mollusks. Preferably, the sialidase is isolated by processing crude oyster hepatopancreas to form a crude extract of the sialidase enzyme. The crude enzyme is further concentrated and refined utilizing sequential column chromatography fractogel EMD DEAE-650...
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