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To explore the dynamics of biomolecules, tracing the kinetics of photo‐induced chemical reactions via the triplet excited state (T1) of probe molecules offers a timescale that is about 106 times wider than via the singlet excited state (S1). Using cyclooctatetraene (COT) as a triplet energy acceptor and at the same time as a photostabilizer, the triplet–triplet energy transfer (TTET) kinetics governed...
Single‐molecule fluorescence resonance energy transfer (smFRET) is a powerful tool to investigate the dynamics of biomolecular events in real time. However, it requires two fluorophores and can be applied only to dynamics that accompany large changes in distance between the molecules. Herein, we introduce a method for kinetic analysis based on control of fluorescence blinking (KACB), a general approach...
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