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In this study, RNAi technology was used to silence the gene rstA in Aeromonas hydrophila. The strain rstA‐RNAi displayed significant decrease in intracellular survival compared with that of the wild‐type strain B11. Transcriptome analysis explored that the expression of some important anti‐stress protein genes was significantly upregulated in rstA‐RNAi compared with the wild‐type strain, while the...
Survival in host macrophages is an effective strategy for pathogenic bacteria to spread. Aeromonas hydrophila has been found to survive in fish macrophages, but the mechanisms remain unknown. In this paper, the roles and possible mechanisms of IcmF in bacterial survival in fish macrophages were investigated. First, a stable silencing strain icmF‐RNAi was constructed by shRNA and RT‐qPCR confirmed...
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