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The production of hGM-CSF was investigated in both a flask and a 5-l bioreactor, using transgenic Nicotiana tabacum suspension cells. While the maximum cell density and secreted hGM-CSF in the flask were 15.4gl −1 and 6.5μgl −1 , respectively, those in the bioreactor were 15.6gl −1 and 7.6μgl −1 . No detectable growth inhibition, shorter production of hGM-CSF and reduced...
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