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OBJECTIVETo purify human muscle‐derived cells (hMDCs) that could be used for managing urinary incontinence, erectile dysfunction and for bladder reconstitution.
MATERIALS AND METHODShMDCs isolated by a modified preplate technique (MPT) from skeletal muscles of limbs were purified by CD34 antibody and magnetic Dynabeads cell selection system (MDCSS). The growth‐doubling time of the hMDCs and purified...
OBJECTIVETo investigate whether CD34+ purified human muscle‐derived cells (hMDCs) are capable of multiple lineage differentiation.
MATERIALS AND METHODSThe hMDCs were isolated from human skeletal muscle and purified using a CD34+ cell selection system (Dynal Biotech, Oslo, Norway). Adherent populations of cells were expanded in culture and cell differentiation was induced using different kinds of...
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