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A micrometer-sized giant vesicle is studied by extending from the inside by using dual-beam optical tweezers in order to measure mechanical properties such as bending rigidity and surface tension of the membrane. As a micrometer-sized vesicle is extended, its shape gradually changes from a sphere to a lemon-shape, and discretely the lemon-shape deforms into a shape of a tube beside a sphere or a lemon...
Microorifice-based fusion makes use of electric field constriction to assure high-yield one-to-one fusion of selected cell pairs. The aim of this paper is to verify feasibility of high-yield cell fusion on a microfluidic chip. This paper also examines viability of the fusant created on the chip. We fabricated a microfluidic chip to fuse selected cell pairs and to study postfusion behavior. We used...
This paper presents and discusses method of tilt controlled optical tweezers. The method can control the orientation of an anisotropic object such as a living cell, sub-mum-thick fiber and mum-sized silica bar. Since the tilt control method can be easily added to a conventional optical tweezers system by use of a single focused laser beam with a single-mirror control, this method would be applicable...
Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe both solution-phase and surface-bound...
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