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Z-Aspartame (Z-APM) was obtained using an aqueous reaction at pH 6.0 based on thermodynamic control and in situ product removal. The lower pH reaction medium led to higher solubilities of the two substrates, l-PheOMe and Z-l-Asp and dramatically lowered the solubility of the product. The higher pH stability of the protease PT121 and the mutant Y114S enabled the pH modulation of the reaction medium...
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