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In this work, aqueous biphasic systems (ABSs) composed of ionic liquids (ILs) and deep eutectic solvents (DESs) were developed and utilized to efficiently extract DNA for the first time. Four kinds of ILs/DESs were mainly constituted by betaine/carboxylic acid and betaine/carbohydrates respectively. While another DES ([TBAB][PPG400]) was formed by polypropylene glycol 400 (PPG 400) and tetrabutylammonium...
A novel ternary hydrosulphonyl-based deep eutectic solvent (THS-DES) comprised of choline chloride/itaconic acid/3-mercaptopropionic acid (molar ratio 2:1:1) was firstly synthesized. The composition, property and microscopic structure of the new magnetic adsorbent (THS-DES@M-GO) based on the THS-DES modified the magnetic graphene oxide (M-GO) was characterized by the system. Magnetic solid-phase extraction...
The interference signals and overlapped peaks are common phenomena in fluorescence determination, which seriously influence the accuracy and reliability of analytical results. In this paper, Krawtchouk image moment method was introduced to the analysis of fluorescence three-dimensional (3D) spectra and applied to the quantitative analysis of three drugs including nicotinic acid, metoprolol and amlodipine...
Immunoassay for cancer biomarkers plays an important role in cancer prevention and early diagnosis. To the development of immunoassay, the quality and stability of applied antibody is one of the key points to obtain reliability and high sensitivity for immunoassay. The main purpose of this study was to develop a novel immunoassay for ultrasensitive detection of cancer biomarker alpha-fetoprotein (AFP)...
A novel procedure for selective extraction of malachite green (MG) from fish samples was set up by using magnetic molecularly imprinted polymers (MMIP) as the solid phase extraction material followed by electrochemiluminescence (ECL) determination. MMIP was prepared by using Fe3O4 magnetite as magnetic component, MG as template molecule, methacrylic acid (MAA) as functional monomer and ethylene glycol...
In this study, a simple and sensitive aptamer-based fluorescence method for the detection of Kanamycin A by using gold nanoparticles (AuNPs) has been developed. In this assay, AuNPs were utilized as DNA nanocarrier as well as efficient fluorescence quencher. In the absence of Kanamycin A, dye-labeled aptamer could be adsorbed onto the surface of AuNPs and the fluorescence signal was quenched. In the...
In this study, the surface enhanced Raman spectra (SERS) of two prohibited veterinary drugs, metronidazole (MNZ) and ronidazole (RNZ), have been acquired, and compared to the theoretically calculated spectra using density function theory (DFT). The experimental Raman and SERS spectra of MNZ and RNZ exhibit high resemblance with the DFT calculations. SERS detection of MNZ and RNZ from standard solutions...
A novel electrochemical sensor based on molecularly imprinted polymer film has been developed for aspirin detection. The sensitive film was prepared by co-polymerization of p-aminothiophenol (p-ATP) and HAuCl 4 on the Au electrode surface. First, p-ATP was self-assembled on the Au electrode surface by the formation of Au–S bonds. Then, the acetylsalicylic acid (ASA) template was assembled...
A comprehensive metabonomic method, in combination with fingerprint analysis and target analysis, was performed to reveal potential mechanisms of berberine action in the treatment of patients with type 2 diabetes and dyslipidemia. Serum samples of 60 patients before and after treatment with either berberine or placebo were collected. Ultra-performance liquid chromatography and quadrupole time-of-flight...
A novel electrochemical biosensor is described for detection of breakpoint cluster region gene and a cellular abl (BCR/ABL) fusion gene in chronic myelogenous leukemia (CML) by using thiolated-hairpin locked nucleic acids (LNA) as the capture probe. The hairpin LNA probe was immobilized on the nanogold (NG)/poly-eriochrome black T (EBT) film-modified glassy carbon electrode (GCE). The immobilized...
An ultra fast liquid chromatography coupled with IT-TOF mass spectrometry (UFLC/MS-IT-TOF) metabonomic approach was employed to study the plasma and urine metabolic profiling of atherosclerosis rats. Acquired data were subjected to principal component analysis (PCA) for differentiating the atherosclerosis and the control groups. Potential biomarkers were screened by using S-plot and were identified...
This paper focuses on investigating the structure parameters of antibiotics which have decisive influence on the interactive mode between antibiotics and DNAs. The analytical results of Best Prediction Set Support Vector Machine Method (BPSSVM) show that the number of N atoms, the number of double bonds, the number of lone-pair electrons, the molar refractivity and the molecular radius are the main...
In this paper, 24 parameters (descriptors) that can influence the interaction of selected antibiotic compound (antibiotics) and DNA have been investigated. Principal components analysis (PCA) and hierarchical cluster analysis (HCA) were employed in order to reduce dimensionality and investigate which subset of variables could be more effective for classifying the antibiotics according to their binding...
A new resonance light scattering (RLS) assay of proteins is presented. In the citric acid-NaOH (pH 2.35) buffer, the RLS of Resorcinol yellow (RY)-protein system can be greatly enhanced by addition of nonionic surfactant OP, owing to the interaction between OP and RY-protein. The enhanced RLS is in proportion to the concentration of proteins in the range 0.02-4.0 μg ml -1 for both bovine...
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