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A method of the sensitivity enhancement for the wavelength modulation surface plasmon resonance immunosensing is described using an antibody–antigen coupling assay. The sensor surface was prepared by self-assembling monolayer (SAM), and regenerated by rising with citrate buffer. The human factor B (Bf) showed a good response in the concentration range 0.50–20.00μg/ml in direct assay. However, Bf had...
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