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Microbial transformation of betulonic acid by Nocardia sp. NRRL 5646 in preparative scale resulted in the isolation of one unexpected asymmetric α-hydroxylation product, methyl 2α-acetoxy-3-oxo-lup-20(29)-en-28-oate, and one known compound methyl 3-oxo-lup-20(29)-en-28-oate. The structures of metabolites were elucidated unambiguously by ESI-MS, 2D-NMR spectroscopy. This is the first successful microbial...
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