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Electron spin resonance spectroscopy was applied to determining the antioxidant capacity of eight thiol-containing compounds, including reduced glutathione, N-acetyl-L-cycsteine, methimazole, captopril, and tiopronin with one thiol group, 1,4-dithioerythritol and 2,3-dimercapto-1-propanol with two thiol groups, as well as L-cystine with no free thiol group. Cu2+ ion gives an electron spin resonance...
Since controlling hydrogen ion concentration (i.e. pH) is fairly important in various fields, developing a facile pH detection method is highly desired. Owing to the superior optical properties, semiconductor quantum dots (QDs) have been developed as a promising alternative for organic fluorophores in fluorescence analysis. In this study, a hydrophilic p-aminothiophenol (pATP) capped CdSe/ZnS QDs...
Paclitaxel is a broad-spectrum anti-cancer drug by targeting microtubulin. However, multidrug resistant (MDR) makes its clinical application more difficult and results in failure of chemotherapy. Tetrandrine as a potential multidrug resistant modulator could be combined with other anti-cancer drugs. In this study, ultra-performance liquid chromatography (UHPLC) combined with quadrupole time-of-flight...
Photodynamic therapy (PDT) is an emerging technique to induce cancer cell death. However, the tumor specificity, cellular uptake and biodistribution of many photosensitizers urgently need to be improved. In this regard, we show here that the integrated nanoassemblies based on G-quadruplex DNAs (GQDs)/protoporphyrin IX (PPIX) can serve as a synergistic platform for targeted high-performance PDT. In...
Herein, one multifunctional AS1411-functionalized fluorescent gold nanoparticles (named NAANPs) is synthesized and successfully applied for both targeted cancer cell imaging and efficient photodynamic therapy (PDT). The NAANPs are obtained by functionalizing the gold nanoparticles with AS1411 aptamer and then bound with one porphyrin derivative N-methylmesoporphyrin IX (NMM). Using HeLa cells over...
Herein, folic acid (FA) conjugated with AuNPs were introduced into the cancer cell imaging via the specific interaction between FA and the folate receptor on the cell surface. FA protected gold nanoparticles (AuNPs) was synthesized and labeled with fluorescein isothiocynate (FITC) to form the FITC–FA–AuNPs (FFANPs). As over-expressed folic acid receptor in some cancer cells and folic acid can specifically...
Herein, a novel kind of silver nanocluster is synthesized simply by mixing G-quadruplex template with silver ions and reduction reagent (NaBH 4 , here). AS1411 (a G-quadruplex that can bind nucleolin overexpressed in cancer cells) is used as the main model template to prove the synthesis protocol and its potential application. We used fluorescence assay, CD, MALDI TOF MS, and TEM to characterize...
A simple method based on simultaneous microwave-assisted derivatization and ionic liquid-based dispersive liquid–liquid microextraction (IL-based DLLME) is proposed for the derivatization, extraction and preconcentration of formaldehyde in beverage samples prior to the determination by high-performance liquid chromatography (HPLC). Formaldehyde was in situ derivatized with 2,4-dinitrophenylhydrazine...
In this paper, we first reported the viable but nonculturable (VBNC) cells used for fabricating biosensor. The organic–inorganic hybrid material composed of silica and the grafting copolymer of poly(vinyl alcohol) and 4-vinylpyridine (PVA-g-P(4-VP)) was used to immobilize microbial cells for biosensor fabrication. The VBNC cells were formed after the hybrid material dried, showing the cell walls were...
Ultrasonic nebulization extraction (UNE) coupled with on-line gas chromatography (GC) was proposed for the determination of trans-anethole in fruits of Illicium verum Hook. f. and Foeniculum vulgare Mill. The extraction was performed in a common self-made extraction system. In the UNE the analyte was transferred and enriched from the solid sample to gas phase. The sample gas containing analyte obtained...
A plasmon resonance scattering (PRS) method for chlorine dioxide is reported based on the oxidization of silver nanoparticles (NPs) by it, in pH 9.1 ammonia–ammonium nitrate buffer solutions. Silver NPs exhibit strong PRS signals at 470nm, and can be oxidized by ClO 2 , which results in PRS quenching at 470nm. It was found that the PRS quenching intensity is proportional to the concentration...
A new method of combining low-temperature Shpol'skii effect with non-linear variable-angle synchronous fluorescence spectrometry (L-NLVASFS) has been proposed to increase spectral resolution. This coupled method was applied successfully to the simultaneous identification and quantification of some polycyclic aromatic hydrocarbons (PAHs) in mixtures, which cannot be determined by non-linear variable-angle...
In Na 2 HPO 4 –citric acid buffer solution, Cl 2 can oxidize I − to form I 2 and then it reacts with excess I − to form I 3− . The I 3− combines respectively with rhodamine dyes, including rhodamine B (RhB), butyl rhodamine B (b-RhB), rhodamine 6G (RhG) and rhodamine S (RhS), to form association particles which give stronger...
A reusable piezoelectric immunosensor with amplified sensitivity has been developed for the detection of ceruloplasmin (CP) in human serum. The quartz crystal microbalance (QCM) was deposited with plasma-polymerized n-butyl amine film with the surface topology further characterized by using atomic force microscopy (AFM). Anti-ceruloplasmin antibody (CP-Ab) was electrostatically adsorbed on the PPF-modified...
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