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SGLT1 as a Na + /glucose cotransporter is inhibited by phlorizin, a phloretin 2 ' -glucoside that has strong interactions with the C-terminal loop 13 (residues 541-638). Here we investigated the effect of a partial substitution of glycerol for water in the medium on the stability and phlorizin-binding function of loop 13 using fluorescence spectroscopy. Increasing the glycerol concentration...
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