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BACKGROUNDInulin fructotransferase (IFTase) catalyzes inulin conversion to difructose anhydride (DFA III), which is a natural low‐calorie sweetener. Although heterologous expression of IFTase was achieved in Escherichia coli, the extracellular enzyme activity was very low, which limited the commercialization of IFTase.
RESULTSActive IFTase of about 43 kDa molecular mass of subunit was extracellularly...
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