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Here a conducting polymer-based cell immobilization method was presented to form controllable olfactory cellular network on microelectrode array. PAIN conducting polymer was electropolymerized on the surface of microelectrode array in a sequence in order to modify the defined microelectrode. Different antibodies can be linked covalently onto defined sites for the effective capture and immobilization...
Mammalian taste system can detect and distinguish various taste signals presented by different tastants using various kinds of taste bud cells. This study described a novel bioelectronic sensor in which taste bud cells were used as biotransducer for detecting bitter signals to investigate taste signal transduction. Using a light-addressable potentiometric sensor (LAPS) as sensing chip to monitor both...
This study focuses on the odor detection with “in vivo bioelectronic nose”. Still, high specific sensing elements and improved shelf-life are highly desired for the construction of biosensors. So we utilized the Green fluorescent protein (GFP) genetically labeled mouse M72 as sensing components to improve the specificity. In addition, long-term in vivo electrophysiological monitoring from M72 olfactory...
Okadaic acid (OA) is typical diarrhetic shellfish poisoning (DSP) toxins, and many detection methods were established for its detection. However, the drawbacks of these methods hamper their wide applications. Here, human bronchial epithelial cell lines (BEAS2B) were selected as the sensitive elements to fabricate the high-sensitive cell-based impedance biosensor to detect the cytotoxicity induced...
We present a new surface acoustic wave (SAW) chip for odorant detection by the using of olfactory receptors as molecular sensors. The molecular sensors were originated from the expression of ODR-10 in human breast cancer MCF-7 cells, which is an olfactory receptor of C. elegances. ODR-10 was then combined with SAW chips to serve as molecular sensors for odorant detection. The resonance frequency shifts...
In this paper, we present a ultra high sensitive (Zeptomole, 10-21) technique to enable the detection of any potential low abundance biomarkers. We demonstrated for the first time the detection of sub 13 nm high-moment magnetic nanoparticle and the implementation of a novel magnetoresistive (GMR) biosensor concept with higher sensitivity and 10 times lower external field in real biomarker sensing...
A novel biosensor based on the platform of neuron- silicon interface was designed and characterized. Taste receptor cells cultured on light addressable potentiometric sensor (LAPS) can recognize sour stimuli as compared with non-acid solutions. A computational model of taste receptor cells was constructed to simulate the ionic currents and action potentials of taste receptor cells, to help decode...
In recent years, quartz crystal microbalance (QCM) plays an important role in many biological detections, and the enhancement of a single strain DNA probe linked to the sensor surface is of crucial importance in DNA molecule recognition. By means of nanogold modification and mass amplification, a QCM biosensor for Escherichia coli O157:H7 DNA detection was explored and developed in this work. As a...
A DNA piezoelectric biosensing method for real-time detection of E. coli O157:H7 in a circulating-flow system was developed in this study. The thioled surface of the Au electrode could be immobilized by many inner Au nanoparticles, then more thiolated single-stranded DNA (ssDNA) probes which was specific to E. coli O157:H7 eaeA gene could be fixed through Au-SH bonding. The hybridization was induced...
A rigorous preselection of identified compounds by in vitro cellular screening is necessary prior to using the drug candidates for the further time consuming and parallel monitoring stage. In this paper we introduced an integrated cell based chip which can detect changes of electrophysiological parameters by using microelectronic chips such as light-addressable potentiometric sensors(LAPS), micro-electrodes...
Cells are equipped with a host of receptors that can convert chemical signals into electrical ones. Cell-based biosensors take cells as sensing elements for detecting the intracellular/extracellular microenvironment and some special characteristics of cells selves. This paper presents the design of the cell-based biosensor and optimization of the automatic fluidic system that makes it feasible to...
Olfactory system can distinguish thousands of odors. In order to utilize biological olfactory neurons as sensitive material of electronic nose, this article reports an olfactory neurochip as real bionic technique for odorants detection. Effective cultures of olfactory receptor neurons and olfactory bulb cells on the chip have been achieved. Using light-addressable potentiometric sensor (LAPS) as sensing...
Immunobiosensors were developed for detection of Escherichia coli O157:H7 based on the surface immobilization of monoclone antibodies onto indium tin oxide (ITO) electrodes. The immobilization of antibodies onto ITO chips was carried out by silanization. The effects of epoxysilane monolayer, the antibody layer on the electrochemical properties of the electrode, and the combined target bacteria were...
In this paper, we propose a non-invasive detection method of lung cancer combined with a sort of virtual SAW gas sensors array and imaging recognition method. A patient's breath goes through an electronic nose with solid phase micro extraction (SPME) and capillary column for pre-concentration and separation of volatile organic compounds (VOCs) respectively, a pair of SAW sensors one coated with a...
A novel numerical design of experimental analysis of cell-based sensors was introduced in the paper. In order to increase the signals to noise ratio and improve the performance of the chips, we analyzed cell-based sensors numerically and experimentally. Since the action potential which is about dozens of milliseconds changes quickly, we demonstrated an improved equivalent circuit model. Based on modeling...
Cell-based biosensors incorporate cells as sensing elements that convert a change in immediate environment to signals conducive for processing. It characterizes with high sensitivity, excellent selectivity and fast response. Cell-based biosensors have been implemented for a number of applications ranging from pharmaceutical screening to environmental pollutant detection. This work demonstrate a novel...
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