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This study presents a rapid, specific and sensitive LC–MS/MS assay for the determination of ramipril and ramiprilat in human serum using enalapril as an internal standard (IS). A Waters Atlantis C18 column (2.1mm×100mm, 3μm) and a mobile phase consisting of 0.1% formic acid–methanol (25:75, v/v) were used for separation. The analysis was performed by the selected reaction monitoring (SRM) method,...
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