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Human FIX expression vector pCMVIX was packaged by Effectene™ reagent and injected into mice seminiferous tubules with glass pipettes. The expressional frame of pCMVIX was examined by PCR and Southern blotting among 41 progenies. There were 2 (4%) mice being integrated with hFIX gene into chromosomes. 4.6 ng/mL of hFIX protein was expressed in plasma of one mouse, which was tested by ELISA. We demonstrated...
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