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To investigate the in vivo function of the newly defined DNase I hypersensitive site HS-48 on the whole human α-globin gene cluster, the region containing all the other known 5 hypersensitive sites HS-4 to HS-40 was deleted from a 117 kb bacterial artificial chromosome clone bearing the whole human α-globin gene cluster. Transgenic mice were generated from this construct. The RNase protection assays...
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