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We report four highly informative multiplex PCRs developed from 12 previously described EST-SSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in 12 full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred...
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