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The functional effect of a monoclonal antibody, RA6.3, on mechanoelectrical transduction (MET) of outer hair cells (OHCs) isolated from the adult guinea-pig cochlea was investigated. This antibody was raised by an antiidiotypic approach against amiloride binding sites. RA6.3 irreversibly reduced the receptor current, independent of membrane potential. The time course of the functional block was independent...
Endocytosis in cochlear hair cells was investigated by staining with the vital fluorescent dye FM 1-43, that partitions reversibly into membranes and is trapped in vesicles during endocytosis. The temporal development and spatial distribution of FM 1-43 induced fluorescence was investigated using confocal laser-scanning microscopy. FM 1-43 rapidly and intensely stained cochlear hair cells, leaving...
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