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The PCR-amplified β-subunit of the human chorionic gonadotropin structural gene (βhCG) was cloned under the control of the tac promoter and the heat-labile enterotoxin chain B (LTB) signal sequence (LTBss). βCG was successfully produced, processed and exported to the periplasmic space in Escherichia coli. Expression of βhCG was confirmed by immunoblot analysis using an anti-βhCG polyclonal antibody...
A fusion gene was constructed consisting of heat labile enterotoxin chain B (LTB) of E. coli genetically linked at its C-terminus to the β-subunit of human chorionic gonadotropin in translational fusion, under the control of tac promoter and LTB signal sequence. Expression of the fusion gene (about 5 μg/ml) in E. coli was confirmed by immunoblot analysis using both anti-LTB and anti-βhCG polyclonal...
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