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Physicochemical surface modifications of soft hydrogel layers are beneficial tools for modulating biomimetic substrate properties in cell adhesion studies. Recently, a layered system of a polyacrylamide hydrogel with a monolayer coating of maleic anhydride copolymers was introduced, however, without a detailed study of the chemical coupling mechanism. Herein it is shown that a stable coupling results...
Biomolecule attachment and lateral micropatterning of biomolecular layers are essential techniques to provide in advanced biochemical and cell culture assays. For that purpose, we introduced a versatile, simple and robust method to functionalise standard polystyrene well plates. Free amino groups were generated on the polystyrene surface by low pressure ammonia plasma treatment. Subsequently, thin...
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