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The isolated ε subunit of F 1 -ATPase from thermophilic Bacillus PS3 (TF 1 ) binds ATP [Y. Kato-Yamada, M. Yoshida, J. Biol. Chem. 278 (2003) 36013]. The obvious question is whether the ATP binding concern with the regulation of ATP synthase activity or not. If so, the ε subunit even in the ATP synthase complex should have the ability to bind ATP. To check if the ATP binding to the...
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