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The histones including H2a, H2b, H3 and H4 purified from pig liver tissue were immobilized onto Sepharose 4B to create a histone-Sepharose column. During chromatography of cow milk casein by histone-Sepharose column, two isoforms of prion protein (PrP c ) with 34 and 30kDa molecular mass corresponding to diglycosylated and monoglycosylated PrP c respectively were found to be captured...
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